XB-IMG-132178
Xenbase Image ID: 132178
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FIG. 5. Mesoderm-inducing activity of Xbra3. Embryos were
injected with Xbra3 message at the two-cell stage and RNA was
extracted from caps isolated at the stages indicated and analysed by
RT-PCR. (A) Xbra3 induces mesoderm in an FGF-dependent manner.
Xbra is induced after injection of Xbra3 mRNA or treatment
with FGF in the absence but not in the presence of XFD. (B) Xbra3
induces markers of dorsal mesoderm and neural tissue. While both
Xbra and Xbra3 induce eFGF at stages 12 and 25, only Xbra3
induces cardiac actin and Xnot at stage 25. Induction of NCAM is
seen at stages 12 and 25 only in response to Xbra3. (C) Xbra3
induces the early neurogenic genes geminin and Xngnr-1 in animal
caps isolated from Xbra3-injected embryos at stage 12, whereas
Xbra does not. The cDNA preparations analysed in C are the same
as those analysed in Fig. 7. Image published in: Strong CF et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |