Xenbase Image ID: 132182

FIG. 1. pMesogenin1 encodes a novel bHLH transcription factor. (A) Deduced amino acid sequences of pMesogenin1 from both mouse and Xenopus. The sequences were aligned using the BESTFIT program in a GCG package. Identical amino acids are indicated as red letters. The bHLH domain is blocked. (B) Comparison of bHLH domain of pMesogenin1 to other closely related bHLH proteins in vertebrates. The peptide sequence of the bHLH domain of mouse pMesogenin1 was used to perform a BLAST search. The six top matched sequences were selected and aligned to each other using the CLUSTALW program. Sequences of Neurogenin1, M-Twist, and MyoD were also included in the alignment. Identical amino acids are indicated as a hyphen and gaps are indicated as a period. (C) Schematic diagram showing similarities among pMesogenin1 and its related bHLH genes. The bHLH domain is colored black. Numbers on the boxed areas indicate the percentage of same amino acids in the domain compared to mouse pMesogenin1. (D) Phylogenetic dendrogram of pMesogenin1 and its related bHLH proteins. The peptide sequences of the bHLH domains were analyzed by the Kimura method for distance calculation and the resulting distance matrix data were processed by the UGPMA method in the NEIGHBOR program of the PHYLIP sequence analysis software package to obtain a tree dendrogram. Image published in: Yoon JK et al. (2000) Copyright © 2000. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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