Xenbase Image ID: 132249

Fig. 4. ADMP mRNA injection suppresses dorsoanterior structures. Each panel shows embryos representative of 20 separate experiments. mRNA was injected into one dorsal blastomere at the 4- to 8-cell stage in a volume of 5 nl. (A) Control embryos injected with 100 pg preprolactin mRNA; all embryos are siblings at the same stage (approximately stage 35). (B) Embryos injected with ADMP mRNA. A range of phenotypes displaying varying degrees of ventralization is shown. (C) Embryos injected dorsally with 100 pg ADMP mRNA. All embryos are severely ventralized. (D) Embryos injected ventrally; the phenotypic perturbations are much less pronounced. (E) Frequency histogram of Dorsoanterior Index scores of embryos injected dorsally with 5, 25 and 100 pg of either ADMP or preprolactin mRNA demonstrating clear dose dependence. The mean DAI scores of ADMP and preprolactin-injected embryos differ significantly (P<0.01) at all doses. The data shown are representative of four experiments with comparable numbers of embryos and at least 20 experiments overall. Some variation in sensitivity to injected ADMP message was observed between different batches of embryos. Image published in: Moos M et al. (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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