Xenbase Image ID: 132252

Fig. 7. Downregulation of dorsalizing signals and dorsal markers by ADMP. (A) Stage 10 or (B) stage 17 embryos were assayed for expression of the indicated genes by RT-PCR following injection of 1 ng ADMP message into one blastomere of 2-cell embryos. (C,D) Caps were explanted at stage 8 from embryos injected with ADMP message as above and cultured until sibling embryos reached stages 10.5 and 35, respectively; the indicated markers were then assayed by RT-PCR. Total RNA from pools of 10-20 embryos or caps was the template for cDNA synthesis in each case. Control indicates template from which reverse transcriptase was omitted to confirm absence of contaminating genomic DNA sequences. Separate RNA pools corresponding to the conditions displayed were prepared from each of at least three separate fertilizations; each pool was assayed at least twice for the indicated markers. Image published in: Moos M et al. (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page