XB-IMG-132264
Xenbase Image ID: 132264
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Fig. 5. RNAse protection analyses of XNkx-2.3a and XNkx-2.3b
expression in adult Xenopus tissues: (A,B) Radiolabelled riboprobes
specific for each XNkx-2.3 allele were hybridized to 20 mg of total
RNA from various adult Xenopus tissues. XNkx-2.3 sequences
contained in each probe are diagrammed above each autoradiogram
(for details, see Materials and Methods). The probe for XNkx-2.3b2
also recognizes XNkx-2.3b1. (C) Analytical formaldehyde agarose
gel of RNA samples. RNA samples were monitored for integrity and
quantity on analytical formaldehyde agarose gels before using. 10 mg
of each RNA were loaded per lane. P, radiolabelled probe alone.
These probes include the XNkx-2.3 sequences as diagrammed, plus
vector sequences, resulting in a radiolabelled probe of greater length
than the fully protected XNkx-2.3 sequences; t, tRNA control lane; H,
heart RNA; I, intestine RNA; K, kidney RNA; Li, liver RNA; Lu,
lung RNA; Pa, pancreas RNA; Sk, Skeletal muscle RNA; Sp, spleen
RNA; St, stomach RNA. Image published in: Evans SM et al. (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |