XB-IMG-132265
Xenbase Image ID: 132265
|
Fig. 6. RNAse protection analyses of XNkx-2.3 and XNkx-2.5
expression during Xenopus embryonic development. Radiolabelled
probes for each XNkx-2.3 allele (A,B) and for XNkx-2.5 (C) were
hybridized to 20 mg of total RNA extracted from staged Xenopus
embryos (Nieuwkoop and Faber, 1967). RNA samples were
monitored for integrity and quantity on analytical formaldehyde
agarose gels before using. The protected RNA species as indicated
by arrowheads in each figure correspond to full protection of probe
sequences complementary to each cDNA. For probe details, see
Materials and Methods. P, probe alone, including vector sequences
and regions complementary to each cDNA; t, tRNA control; stage 9-
38, RNA from stages 9 through 38 embryos. Image published in: Evans SM et al. (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |