XB-IMG-132267
Xenbase Image ID: 132267
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Fig. 8, Transverse sections from embryos stained by wholemount
in situ for XNkx-2.3 and Xtwist RNA expression. For
experimental details, refer to Materials and Methods. ec,
ectoderm; en, endoderm; ey, eye anlage; h, heart region; m,
mesoderm; my, myocardium of early heart tube; nc,
branchial arch neural crest; ng, neural groove; ph, pharynx.
Embryos were viewed and photographed using Nomarski
optics. In C-E, the outline of each section and the pharynx
are shown with dotted lines to facilitate orientiation. Dorsal
is at the top. (A) Anterior transverse section from a stage 16
embryo stained for XNkx-2.3 RNA expression. Note
staining in mesoderm, and faint staining in the endoderm.
(B) Transverse section from same embryo as in A, but
further posterior. Note staining in mesoderm and stronger
staining of endoderm than in A. (C) Transverse section at
the level of the eye anlage of stage 30 embryo stained for
XNkx-2.3 expression. Staining is observed in the
pharyngeal endoderm. (D) Transverse section just posterior
to that shown in C, indicating staining for XNkx-2.3 in
pharyngeal endoderm and in myocardium. (E) Transverse
section of stage 30
embryo stained for
Xtwist RNA
expression. The
neural crest
invading the
pharyngeal
mesoderm is
stained for twist.
This staining
pattern was never
observed for
embryos stained
for XNkx-2.3
expression. The
endoderm and the
heart do not stain
but their position
is shown to
facilitate
comparison with
D. Image published in: Evans SM et al. (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |