XB-IMG-132314
Xenbase Image ID: 132314
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FIG. 12. Suppression of autonomous neuralization in dissociated ectoderm cells by blocking FGF signaling with DXFGFR-4a. (A) Effects of
overexpression of DXFGFR-4a and DXFGFR-1 in ectoderm cells on their neuralization by prolonged dissociation. DXFGFR-4a or DXFGFR-1
mRNA was injected into four animal blastomeres at the eight-cell stage (300 pg/blastomere). The injected or uninjected embryos were incubated
until stage 10 when ectoderm cells were isolated and subjected to dissociation for 30 or 90 min before reaggregation and culture. The transcription
levels of panneural markers NCAM and Nrp-1 (left panels), anterior neural markersBF-1 and Rx-1 (middle panels), epidermal marker Keratin (top
right panels), and cement gland marker XAG-1 (bottom right panels) in these cultures were analyzed by quantitative RT-PCR assay as in Fig. 7.
Autoradiographs are shown of RT-PCR products of the marker transcripts coamplified with EF1a transcript. (B) Quantitative assessment of
effects of injected DXFGFR-4a and DXFGFR-4a mRNAs. Each RT-PCR product shown in (A) was quantified as in Fig. 5B. Values were normalized
to EF1a expression as in Fig. 7B and presented as percentages of the maximum values of the ratio in each histogram for NCAM (top left), Nrp-1
(bottom left), BF-1 (top middle), Rx-1 (bottom middle), Keratin (top right), and XAG-1 (bottom right). Image published in: Hongo I et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |