XB-IMG-132369
Xenbase Image ID: 132369
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FIG. 1. (a) Sequence of the Xnr3 promoter from the end of the cDNA to an upstream PstI site located 257 bp upstream. Numbering is
relative to the end of the previously isolated Xnr3 cDNA (Smith et al., 1995). End points of a series of exonuclease III deletions are
indicated with an asterisk (*) above the last base pair of the sequence. Wnt responsive elements, WRE1 and WRE2, are underlined. (b)
Deletion analysis of the Xnr3 promoter. The results from one representative assay are shown in which a selection of the truncated
promoters were tested. The wnt inducibility of plasmids was calculated as the amount of luciferase activity present in extracts from
gastrulae injected with the plasmid along with Xwnt8 mRNA, divided by the amount of luciferase activity present in extracts from
gastrulae which were injected with the plasmid alone. The results from this and other experiments show that the 5* end of a wntresponsive
element lies between position -160 and position -142. Image published in: McKendry R et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |