Xenbase Image ID: 132370

FIG. 2. (a) Sequence of linker-scanning mutations of the Xnr3 promoter. Eight- to 10-bp stretches (between 0141 and 063) of the Xnr3 promoter in p160Xnr3 were replaced with a NotI site as shown. The replacement sequences are in lowercase letters and surrounding sequences are shown for orientation. These new plasmids, p160LS3 through p160LS11, were tested for their wnt inducibility. (b) Wnt responsiveness of linker-scanning mutants of the Xnr3 promoter. Two representative assays which show that mutations in either p160LS3 or p160LS8 reduce the wnt inducibility of the promoter about 10-fold are shown. Two independent isolates of each mutant construct were tested at least twice and in separate experiments. (c) Mutational analysis of WRE1. Mutation of 5*-ATTA to 5*-AGGA at position 0143/4, significantly reduces the wnt inducibility of the Xnr3 promoter. (d) The b-catenin response of the Xnr3 promoter is dependent on WRE2. The plasmids p160Xnr3 and p160LS8 were tested for their ability to respond to Xwnt8 and bcatenin. The response of the promoter to b-catenin also requires the sequences removed by the LS8 replacement and so is dependent on WRE2. Image published in: McKendry R et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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