XB-IMG-132372
Xenbase Image ID: 132372
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FIG. 4. Mammalian LEF-1 can bind to the sequence from -125
to -63. Embryos were injected with 1 ng LEF-1 mRNA, 1 ng LEF-
1DN mRNA, or 1 ng Xwnt mRNA at the single-cell stage. They
were harvested at gastrulation and extracts were incubated with a
radiolabeled copy of the sequences between -125 and -63 of the
Xnr3 promoter. A novel shifted complex is detected in extracts
from embryos injected with LEF-1 mRNA (lane 2). A complex of
greater mobility is detected in extracts from embryos injected with
LEF-1D56 mRNA (lane 3), indicating that mammalian protein can bind
these sequences directly. Image published in: McKendry R et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |