XB-IMG-132373
Xenbase Image ID: 132373
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FIG. 5. (a) Embryo extracts contain proteins which bind WRE1
specifically. Extracts were made from midgastrula embryos (stage
11) and mixed with a radiolabeled probe containing sequences from
0160 to 0129 of the Xnr3 promoter and complexes separated by
electrophoresis. Lane 1 contains no competitor, whereas 100-fold
excess of unlabeled competitor DNA was added to the binding
reactions loaded in lanes 2, 3, and 4. Competitor DNAs are wildtype
sequences from the Xnr3 promoter between 0160 and 0129
(lane 2), or 0160 to 0129 with a 2-bp point mutation 5*-ATTA to
5*-AGGA (lane 3), or carrying the same sequence replacement as
is present in p160LS3 (lane 4). (b) A developmentally regulated
complex binds sequences between 0160 and 0104 specifically. Embryos
were injected at the single-cell stage with a-amanitin (an
inhibitor of RNA polymerase II transcription) or left uninjected.
Extracts were then prepared from embryos at different stages of
development as indicated, and a gel retardation assay was performed
using the sequences between 0160 and 0104 as probe. Image published in: McKendry R et al. (1997) Copyright © 1997. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |