Xenbase Image ID: 132505

FIG. 4. Rhodopsin transcript analysis. A, Northern blot. 2 mg of Xenopus adult total brain (lane 1) and retinal (lane 2) RNA was resolved on a denaturing agarose gel, and rhodopsin transcripts were detected by hybridization with a cDNA probe (nucleotides 315–856). The single 1.7-kb retina-specific message is indicated (arrow). Arrowheads represent molecular weight markers of 9.4, 7.5, 4.4, 2.4, and 1.4 kb from top to bottom. B, primer extension. Primer extension of total RNA with radioactively labeled antisense primer P10 is shown. Reactions were carried out using 5 mg of total retinal RNA (lane 1) and with a 50-fold excess of unlabeled P10 (lane 2) or total brain RNA (lane 3). A sequencing ladder using the same primer is also shown. The underlined nucleotide indicates the major transcription start site and minor start sites are indicated by smaller asterisks. Image published in: Batni S et al. (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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