XB-IMG-132506
Xenbase Image ID: 132506
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FIG. 5. A, Southern analysis. Southern blot of Xenopus genomic DNA
undigested (lane 1) or digested with SacI (lane 2), EcoRI (lane 3), or
BamHII (lane 4), hybridized with an exon 1 probe (nucleotides 1–444)
and washed at high stringency. B, PCR of intron 1 using genomic DNA.
PCR using primers P1 and P12 (see Fig. 2) were used to amplify intron
1 from genomic DNA (lanes 1–3 and 5) or from genomic clone lgopRI
(lanes 6–9). Amplifications were carried out with P1 and P12 (lanes 1
and 6), P1 (lanes 2 and 7), P12 (lanes 3 and 8), no primers (lanes 5 and
9), and primers alone, no genomic DNA (lane 4). The sizes of the four
products found in lane 1 are 367, 500, 550, and 650 bp. Image published in: Batni S et al. (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |