Xenbase Image ID: 132508

FIG. 7. Rhodopsin upstream sequences direct transient expression of luciferase in Xenopus embryos. A, the luciferase reporter constructs are diagrammed with the luciferase gene (luc) transcribed from left to right. Solid boxes indicate genomic sequences from XOP1 and GL2 is the (promoterless) control plasmid. B, Luciferase levels obtained from transient expression experiments. Stage 26/27 embryos were dissected and treated with trypsin in the presence of EDTA prior to lipofection (Experiments A and C). Additional embryos were dissected and the head epidermis was manually removed prior to trypsinization and lipofection (Experiment B). Embryonic tissue was incubated to the equivalent of stage 42/43 and assayed for luciferase activity. Activities are presented as RLU/embryo, where 1 pg of luciferase 5 85,000 RLU. Early stage blastomeres (8-cell or 32-cell, Experiment D or E, respectively) were injected with plasmid and cultured to stage 42, when luciferase levels were determined. Image published in: Batni S et al. (1996) Copyright © 1996. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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