XB-IMG-132546
Xenbase Image ID: 132546
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Fig. 4. Localization of Xwnt-8b transcripts in neurula and tailbud stage embryos by whole-mount in situ hybridization. (A) Neurula (st. 21), and
(B) sagittal section of neurula stage embryo hybridized with a digoxigenin-labeled antisense Xwnt-8b probe showing signal at junction between
prospective prosencephalon and mesencephalon (arrowheads). (C) Low (left side) and high (right side) magnification views of whole tailbud
(st. 33) embryos hybridized with antisense Xwnt-8b probe. Staining of dorsal diencephalon (black arrowhead), forebrain-midbrain border
(white arrowhead) and dorsal mesencephalon (white arrow) is indicated. Embryo on right was stained overnight to visualize midbrain staining.
All of the blue signal with the exception of that indicated by arrows was also observed in embryos hybridized overnight with a sense probe
(data not shown) and is thus nonspecific staining. (D) Tailbud (st. 33) embryo hybridized with sense Xwnt-8b probe. Low (E,G) and high (F,H)
magnification views of sagittal sections of tailbud (st. 33) embryos showing Xwnt-8b expression in the dorsal diencephalon (arrowheads). Image published in: Cui Y et al. (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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