Xenbase Image ID: 132627

FIG. 7. A strip of ventral meso-ectoderm from TRITC-dex-labeled embryos was transplanted into unlabeled hosts to see if cells rearrange. Donor embryos were injected with TRITC-dex in all cells of the 4-cell stage, at stage 16 strips of ventral meso-ectoderm were transplanted from donors to hosts, and at stage 27 hosts were fixed and labeled cells examined on a confocal microscope (A). Parasagittal (B) and cross section (C) of a neurula fixed shortly after transplantation to show the size, shape, and position of the labeled cells. Higher magnification view of the embryo in B and C showing the rectangular shape and coherence of the initial graft (D). Confocal images of parasagittal (E) and cross section (F) of a tailbud embryo showing the more ventral location of the labeled mesodermal cells. Higher magnification view of the tailbud embryo in E and F showing labeled mesodermal cells separated from the bulk of the transplant in A-P dimension (G, arrows) and some ectoderm cells separated from the bulk of the transplant in the D-V dimension (G, arrowhead). Autofluorescence of yolk platelets makes unlabeled cells of the host visible. Anterior is to the left and dorsal is up in B, D, E, and G. Dorsal is up in C and F. Scale bar in B applies to B, C, E, and F and is 500 mm. Scale bar in D applies to D and G and is 150 mm. Image published in: Larkin K and Danilchik MV (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

Return to previous page