XB-IMG-132639
Xenbase Image ID: 132639
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FIG. 5. Ectopic targeted expression of SRG4 results in an expanded pattern of Nkx2.5 transcription. Examples are shown of embryos
injected at the 8-cell stage into one left-side dorsal–vegetal blastomere with RNA encoding control LacZ RNA, mtSRG4, or SRG4, as
indicated. Embryos were fixed at stage 26 and analyzed by whole-mount in situ hybridization for Nkx2.5 transcripts; processed embryos
are viewed from the ventral side, anterior is up. The control embryo is slightly older so the right (R) and left (L) sides of the primitive heart
tube are fusing at the midline. Compared to the symmetric pattern seen here or in the prefusing primordia of the mtSRG4-injected embryo,
the SRG4-injected embryos display an expanded domain of Nkx2.5 transcription in the left primordium (arrows). (b) An embryo injected
in the left blastomere with SRG4 RNA was processed as in (a), and sections were taken through the region of the fusing primordia. Shown
are four sections taken from progressively more posterior regions (left to right). Note that in the most anterior regions the Nkx2.5 pattern
is not significantly changed, but left-side enhanced transcripts are detected in more posterior sections (arrows). Image published in: Jiang Y et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |