Xenbase Image ID: 132716

FIGURE 3. Ventralizing activity observed in stage 27 tadpoles following injection of dorsal blastomeres of Xenopus (two- and four-cell) embryos with mRNA encoding wild type or mutant GDF5 and combinations of various SPCs. A, control, sham-injected embryos. B, embryos injected with wild type GDF5 (60 pg) alone. C, embryos injected with GDF5 Lys → Arg mutant (60 pg). D, DAI distribution of embryos injected with GDF5-wild type alone (60 pg), GDF5-wild type (60 pg) + Furin (300 pg) or SPC6 (300 pg) GDF5-wild type (60 pg) + Furin (150 pg) + SPC6 (150 pg) or GDF5 Lys → Arg mutant (60 pg). Injection with Furin (150 pg) + SPC6 (150 pg) only was included as a negative control. The DAI of the embryos was scored at stage 27, and the percentage of embryos having a DAI score <5 for each treatment is shown. Numbers of embryos examined (n) are indicated above each column. Embryos with a DAI of 0 lack dorsal structures completely, and those with a DAI of 5 are normal. Similar results were obtained in three separate experiments. Image published in: Thomas JT et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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