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Supplemental Figure 2. Dorso-Anterior Index (DAI) distribution of embryos injected with GDF5-wt alone (60pg), GDF5-wt (60pg) + Furin (300pg) or SPC4 (300pg) or SPC6 (300pg), GDF5-wt (60pg) + Furin (150pg) + SPC4 (150pg), GDF5-wt (60pg) + Furin (150pg) + SPC6 (150pg) or GDF5-wt (60pg) + SPC4 (150pg) + SPC6 (150pg). The DAI of the embryos was scored at stage 27, and the percentage of embryos having a DAI score of < 5 for each treatment is shown. Numbers of embryos examined (n) are indicated above each column. Embryos with a DAI of 0 lack dorsal structures completely, and those with a DAI of 5 are normal. Similar results were obtained in three separate experiments. Image published in: Thomas JT et al. (2006) Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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