Xenbase Image ID: 132737

Fig. 3. Pax3 and Pax3/Zic1 immediate early target genes in the neural crest signature (A) Experimental strategy used to identify Pax3 and Pax3/Zic1 immediate early target genes. (B) List of direct targets belonging to the neural crest signature. (C) Independent validation of a subset of targets by quantitative PCR. Embryos were injected as indicated, either with Pax3GR mRNA, or Zic1GR mRNA, or a combination of both. The animal cap ectoderm was treated with cycloheximide and dexamethasone as described in the text. (D) In vivo validation of the novel Pax3/Zic1 target genes using by either Pax3 or Zic1 knockdown at stage 17. Abbreviations: Log FC, log 2 fold change. Image published in: Plouhinec JL et al. (2014) Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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