Xenbase Image ID: 132949

Figure 5 Delineation of Xvent-2 responsive region. A and B, double stranded oligonucleotides (5′ elongated by a KpnI (forward) or aXhoI (reverse) site) comprising positions +1823 to +1884 (1), +1823 to +1850 (2), +1852 to +1884 (3), and +1823/+1884 deleted from +1842 to +1857 (4) were subjected to gel mobility shifts with the Xvent-2 homeodomain as described in the legend to Fig. 4. Note that there is no shift when both copies of the core motif (underlined) are deleted but that one copy is sufficient for binding. C, fragments were subcloned in front of the −116/+54 basalBMP-4 promoter fused to the luciferase reporter. 20 pg of DNA constructs were co-injected with 500 pg of Xvent-2 RNA into dorsal blastomeres of four-cell stage embryos. Reporter activity of the complete fragment is set as 100%. Note that neither the internal deletion nor the 3′ fragment containing one copy of the core motif can be activated by Xvent-2. Image published in: Schuler-Metz A et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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