Xenbase Image ID: 132951

Figure 7 BMP-4/goosecoid promoter swapping. The goosecoid promoter has recently been shown to contain several Xom (Xvent-2)-binding sites (BS) located between −128 and −226, thereby mediating Xvent-2 caused repression of activin A-stimulated promoter/reporter activity (24). In contrast, the intron 2 enhancer (+1815/+1892) fused to the basalBMP-4 promoter yields a strong activation upon co-injection of Xvent-2. Promoter swapping of corresponding gsc and intron 2 enhancer regions reveals that not the basal promoters, but that the Xvent-2-binding sites are responsible for repression or activation, depending on the context. Reporter activities determined after injection of 20 pg of DNA constructs into dorsal blastomeres at the four-cell stage are set as 100%, the values obtained upon co-injection of 500 pg of Xvent-2 RNA are given as relative percentage. Image published in: Schuler-Metz A et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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