Xenbase Image ID: 132952

Figure 8 Cycloheximide treatment subsequent to Xvent-2 overexpression prevents BMP-4 gene activation. A, four-cell stage embryos were injected with 400 pg of Xvent-2 RNA into both dorsal blastomeres. Cycloheximide treatment was performed as described under “Experimental Procedures.” RT-PCR was performed with total RNA for BMP-4 (upstream primer: 5′-GATTGGCTGTCAAGAATCATGGA-3′; downstream primer 5′: GAACATCTGCAGCAGCGTCACCTCG-3′) and had been adjusted using histone H4 (upstream primer: 5′-CGGGATAACATTCAGGGTATCACT-3′; downstream primer: 5′-ATCCATGGCGGTAACTGTCTTCCT-3′) as an internal control. Image published in: Schuler-Metz A et al. (2000) Copyright © 2000. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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