XB-IMG-132957
Xenbase Image ID: 132957
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FIG. 2. Phenotypic consequences of Pcl1 and other Polycombgroup
overexpression. Embryos were symmetrically injected in
both blastomeres near the dorsal marginal zone at the four-cell
stage with 0.4 ng RNA (A–F). Control (A) and mPcl1 (B–F)
embryos are shown at stage 40. Eyes are small (B, C), absent (D,
E), and occasionally fused (F) and embryos show characteristic
changes in head shape relative to controls. Four-cell embryos
were bilaterally injected with control (b-galactosidase) or mPcl1
RNA, sectioned at Stage 36 (transverse), and stained with
hematoxylin– eosin (G). Note how Pcl1 markedly decreases the
size of the ventricle (v) and expands the surrounding neural
tissue. The positions of the eye (e) and otic vesicle (o) are
indicated. PcG proteins produce similar anterior neural defects
(H). Embryos were injected bilaterally at the four-cell stage with
1 ng of each RNA. At Stages 36–42 embryos were scored (H) for
the distinctive changes in head morphology and eye size shown
in (B–F). At least 30 embryos were analyzed for every condition
in each experiment. Values are the average of three or more
separate experiments. Error bars show SEM. Injected RNAs are
control (b-galactosidase), Xenopus and mouse Pcl1, M33 (mouse
Polycomb1), XBmi-1, and mPh2 (mouse polyhomeotic 2). Image published in: Yoshitake Y et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View |