Xenbase Image ID: 132959

FIG. 4. Pcl1 and other PcG alter expression of En-2 and Krox-20. Expression of En-2 (midbrain–hindbrain boundary) and Krox-20 (hindbrain; rhombomeres 3 and 5) were assayed by whole-mount RNA in situ hybridization at stage 19. Embryos were injected unilaterally at the four-cell stage with either control (GFP; A, D, G) or mPcl1 RNA, plus RNA encoding N-b-galactosidase. b-Galactosidase assay with Red-gal (red product) marks the side of injection (on the left in each panel). Repression of En-2 is dependent upon Pcl1 (B), but uncoupled from repression of Krox-20 (E,H). Posterior shifts of En-2 are also Pcl1-dependent (C), but either coincident with (F) or uncoupled from (I) shifts in Krox-20. PcGs produce similar effects on En-2 expression (J, K). Embryos were injected unilaterally and assayed for En-2 repression (J) and position shift (K) as in (A–I). The percentage of embryos that show the effect and the standard error are given. At least 50 embryos were analyzed in each experiment for every condition. Values are the average of three or more separate experiments. Error bars show SEM. All analyses of En-2 expression changes were performed blind to avoid bias. Image published in: Yoshitake Y et al. (1999) Copyright © 1999. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue en2.L En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 X. laevis Throughout NF stage 19 presumptive midbrain-hindbrain boundary egr2.L EGR-2, Krox-20, krox20, XKr20, XKrox-20 X. laevis Throughout NF stage 19 cranial neural crest rhombomere R3 rhombomere R5 Image source: Published Larger Image Printer Friendly View

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