XB-IMG-133005
Xenbase Image ID: 133005
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Fig. 4. Xbra can induce Zic3 in the mesoderm. (A) Zic3
expression (purple) is shown in the cross-section of the gastrula.
Animal pole is top. The embryos were cut along the broken line
shown. Zic3 is expressed symmetrically in wild-type embryos
(control). Zic3 expression was enhanced in the mesoderm at
stage 10.5 when Xbra (2000 pg) was injected into the lateral side
of one blastomere at the 2-cell stage (Xbra). Xbra-EnR suppressed
the Zic3 expression at the injected side (Xbra-EnR). Coinjected
LacZ expression is indicated in blue. inj, injected side;
D, dorsal side; V, ventral side; bp, blastopore. (B) Reverse transcription
(RT)–polymerase chain reaction (PCR) analysis of animal
cap explants from embryos injected with Xbra (2000 pg)
and/or Xbra-EnR (1000 pg). Zic3 was induced by Xbra whereas
the expression was reduced in the presence of Xbra-EnR. (C)
RT-PCR analysis of Zic3 (100 pg) overexpressed animal cap
explants. Xnr1 and Pitx2 were induced by Zic3, but Xbra was
not. (D) Zic3 was induced by Xbra-GR (400 pg) as early as
20 min after adding dexamethasone. This temporal profile is comparable
to that of Bix1 and Wnt11 which have been shown to be
regulated directly by Xbra. RT-PCR analyses were also carried
out with RNA that had not been reverse transcribed (RT–), RNA
from sibling embryos (Embryo) and uninjected animal cap
explants (uninj). Image published in: Kitaguchi T et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |