XB-IMG-133102
Xenbase Image ID: 133102
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Figure 1 Experimental design for isolating
epidermal inducer cDNA from Xenopus
laevis. A cDNA library was prepared from
Xenopus oocytes at stage 8. For sib selection,
plasmid DNA pools were prepared from the
cDNA library after subdivision and transformation.
RNAs were transcribed in vitro
and microinjected into X. laevis oocytes. After
16 h, the incubation medium was collected
and used for culture of dissociated animal
cap cells. Epidermal inducing activities contained
in the medium were assessed by morphology
of the animal cap cells. Image published in: Nakajima Y et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |