XB-IMG-133136
Xenbase Image ID: 133136
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Fig. 3. Effectiveness and
specificity of antisense
morpholino oligonucleotides
(MOs). Fertilized
eggs were injected with
30 ng of Msx1 (M1) or 10
ng Msx2 (M2) splice-targeted
MOs, which were
then cultured to early neurula (St. 14) along with uninjected embryos (UI)
and processed for Northern blot analysis with Msx1 and Msx2 probes.
Msx1 and Msx2 transcripts were reduced to a baseline of approximately
20% of control levels, as measured by densitometry of X-ray films,
respectively. Neither MO affected the non-homologous RNA, demonstrating
specificity. Ethidium bromide staining of 18S ribosomal RNA is
shown to control for equal RNA loading. Image published in: Khadka D et al. (2006) Copyright © 2006. Reproduced with permission of the Publisher, University of the Basque Country Press. Larger Image Printer Friendly View |