XB-IMG-133137
Xenbase Image ID: 133137
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Fig. 4. Loss of Msx function. Two-cell
stage embryos were injected at a single
site in one blastomere with 15 ng M1, 5
ng M2, along with 250 pg β-galactosidase
RNA as a lineage tracer and cultured to
early neurula (St. 13), then fixed, stained
with X-gal and processed for whole mount
in situ hybridization with probes for the
neural crest marker gene Slug. Dorsal
views, with the hybridization signals represented
by purple color and the lineage
tracer by light blue. The injected side is on
the left in all cases and anterior is towards
the top of the Figure. Both M1 and M2
greatly reduced the expression of Slug.
The Slug expression domains were also
shifted laterally in some cases. Near-normal
levels of Slug expression were rescued
in a majority of embryos by coinjection
of Msx1 mRNA (5 pg, with 5 ng
M2) and Msx2 RNA (10 pg, with 15 ng
M1). An uninjected embryo is shown for
comparison (UI). Image published in: Khadka D et al. (2006) Copyright © 2006. Reproduced with permission of the Publisher, University of the Basque Country Press.
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