XB-IMG-133198
Xenbase Image ID: 133198
|
|
Fig. 3. Differential hybridization analysis by northern blots. To confirm the reliability of the array analysis, 20 selected genes out of
33 positive ones were subjected to the differential hybridization by northern blot. 10 μg of total RNA from animal or vegetal
blastomeres was loaded at each membrane (animal, left lane; vegetal, right lane). Fourteen transcripts were abundant in the vegetal
blastomeres of the 8-cell-stage embryo, while one, XL014k14, was not. Signals of five other genes were not detected in this analysis.
The upper end of each image, except for EF1-α, coincides with the loading start level. The arrowheads represent 28S and 18S
ribosomal RNA. XL087b08 and XL099d22 hybridized with transcripts of at least two different sizes. EF1-α was the internal control for
these RNA. Image published in: Kataoka K et al. (2005) Copyright © 2005. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |