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XB-IMG-133228

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Figure 2. Histology of the adult Xenopus laevis lung. A–G: Longitudinal sections of an adult Xenopus laevis lung including (A) the proximal and (A′) distal regions. B: 20× view of proximal main bronchi showing the epithelial lining and bronchi walls with cartilage plate surrounded by layers of smooth muscle and connective tissue containing a major blood vessel. B′: Detail view of boxed area in (B) showing the epithelial cells in close apposition to capillaries. C: 5× view of the proximal lung showing major septa containing a pulmonary vein and terminal cartilage nodules with smaller septa forming alveoli. C′: 20× view of a cartilage nodule at the end of the major septa. C′: 20× view showing the tip of an alveolar septa. D: 40× view of an alveolar septa tip stained with Mason's Trichrome showing collagen fibers (blue) and muscle fibers (red). E: 5× view of smaller alveoli at the lung periphery. F: 40× view of the blood–air interface in the small alveolar walls. G: In situ hybridization of sftpc (dark purple staining) showing expression in epithelial pneumocytes. H: Brightfield of the adult Xenopus lung (distal portion) cut in a transverse plane immediately after dissection. I,J: Hematoxylin/eosin-stained (I) and (J) Alcian blue-stained transverse sections Abbreviations: alv, alveolar space; at, arteriole; br, bronchiolar space; cap, capillary; cart, cartilage; epi, epithelial cell; pv, pulmonary vein; sm, smooth muscle; sp, septae.Download figure to PowerPoint

Image published in: Rankin SA et al. (2015)

Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons.

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