Xenbase Image ID: 133230

Figure 4. Immunofluorescence analysis of D–V foregut patterning and trachea-esophagus separation in Xenopus. A–O: Confocal immunostaining of the Xenopus foregut showing transverse sections at three positions along the anterior–posterior axis at the indicated stages showing protein expression of Sox2 (blue), Nkx2.1 (red), and FoxF1 (green). P: Transverse section through the E9.5 mouse foregut showing similar expression of Sox2 (blue) Nkx2.1 (red) and FoxF1 (green). Q,R: Transverse sections of the Xenopus foregut at NF36 and NF42 showing P63 expression (white) in the dorsal foregut (Q) and esophagus (R). Abbreviations: dfg, dorsal foregut; eso, esophagus; fge, foregut epithelium; h, heart; lb, lung bud; lpm, lateral plate mesoderm; nc, notochord; s, somite; st, stomach; tr, trachea; ubb, ultimobrachial body; vfg, ventral foregut. Scale bar = 0.1 mm.Download figure to PowerPoint Image published in: Rankin SA et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Gene Synonyms Species Stage(s) Tissue sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 32 endoderm foregut epithelial cell epithelium dorsal sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 33 and 34 endoderm foregut dorsal sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 35 and 36 endoderm foregut epithelial cell esophagus stomach pharynx sox2.L anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 X. laevis Throughout NF stage 42 foregut epithelial cell esophagus stomach nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Throughout NF stage 33 and 34 endoderm foregut ventral lung primordium nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Throughout NF stage 42 trachea foregut epithelial cell lung primordium foxf1.L acdmpv, fkhl5, foxf1-a, foxf1-b, freac1, xfd-13 X. laevis Throughout NF stage 32 lateral plate mesoderm foxf1.L acdmpv, fkhl5, foxf1-a, foxf1-b, freac1, xfd-13 X. laevis Throughout NF stage 33 and 34 lateral plate mesoderm foxf1.L acdmpv, fkhl5, foxf1-a, foxf1-b, freac1, xfd-13 X. laevis Throughout NF stage 35 and 36 mesoderm foxf1.L acdmpv, fkhl5, foxf1-a, foxf1-b, freac1, xfd-13 X. laevis Throughout NF stage 42 mesoderm trachea esophagus stomach smooth muscle nkx2-1.L nkx2.1, titf1, ttf1, Xnkx-2.1, xnkx2-1 X. laevis Throughout NF stage 35 and 36 endoderm foregut lung primordium ventral Image source: Published Larger Image Printer Friendly View

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