Xenbase Image ID: 133233

Figure 7. Activity of the canonical Wnt signaling pathway in the Xenopus foregut. A–L′: Confocal immunostaining of Tg(7xtcf:degfp) transgenic Xenopus tropicalis embryos, which express destabilized enhanced GFP in cell responding to Wnt/β-catenin signaling. Transverse sections show protein expression of Nkx2.1 (red) or Sox2 (blue) and eGFP (green) in the foregut at NF32 (A–B′), NF33 (C–E), NF35 (G–I), and NF42 (J–L). EGFP expression is first detected in the ventral foregut at NF32 before Nkx2.1 (A′). By NF33 Nkx2.1 is activated and the Sox2 expression domain (white bracket) becomes down regulated (B–C′) in the EGFP expressing ventral endoderm. Abbreviations: dfg, dorsal foregut endoderm; eso, esophagus; lb, lung bud; lpm, lateral plate mesoderm; ltg, laryngo-tracheal grove; st, stomach; tr, trachea; vfg, ventral foregut endoderm. Scale bar = 0.1 mm.Download figure to PowerPoint Image published in: Rankin SA et al. (2015) Copyright © 2015. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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