XB-IMG-133336
Xenbase Image ID: 133336
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Fig. 1. Xcat-2 RNA is highly concentrated in the cytoskeletal
fraction of stage VI oocytes. A northern blot of stage VI total
RNA isolated from the IFF ( ) and SF was probed separately
with Xcat-2, Vg1, and histone H3 32P-labeled DNA. In oocytes,
IFF RNA is four-fold more concentrated in poly(A)+ material as
determined by poly(U) hybridization (data not shown). Therefore,
four-fold more SF RNA (16 mg) was loaded to allow comparison
of mRNA concentration between the SF and IFF. Note that like
Vg1, Xcat-2 (0.9 kb) is highly concentrated in IFF RNA whereas
histone H3 RNA, the internal marker for poly(A)+ material, is of
equal concentration in the two fractions. Image published in: Mosquera L et al. (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |