Xenbase Image ID: 133337

Fig. 2. Xcat-2 is localized to the vegetal pole in the stage VI oocyte and embryo. A northern blot of regional RNA extracted from cryostat-sectioned oocytes (King and Barklis, 1985) and embryos was hybridized separately with Xcat-2, Vg1, and histone H3 32P-labeled DNA. On the left blot, total RNA isolated from animal pole (An) and vegetal pole (Vg) fifths of stage VI oocytes is shown. Five-fold less vegetal pole than animal pole poly(A)+ RNA was loaded on the blot (note histone H3 levels), yet Xcat-2 was detected only in the vegetal fifth. On the right blot, each lane contains total RNA (15 mg) isolated from one fifth of 4- to 8-cell embryos sectioned along the animal/vegetal axis (An/Vg). Again, Xcat-2 and Vg1 are found only in the most vegetal fifth (200 mm) of the embryo. Image published in: Mosquera L et al. (1993) Copyright © 1993. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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