Xenbase Image ID: 133362

Figure 4. Depletion of xBMAL1 or xNOCTURNIN results in smaller, disorganized somites with disrupted somite boundaries. Panel A represents the results of analyzing the somite area (uninjected side vs injected) of pairs of somites using NIH Image J. The average ratio of the area of injected to uninjected sides of the embryos analyzed is shown with error bars representing standard deviation. Somites injected with either xBmal1 MO (1 ng) or xNocturnin MO (1 ng) showed a significant decrease in somite area (*) when compared to the control MO (1 ng). Images in panels B-F are oriented with the anterior of the embryo to the left and dorsal up. Panel B shows an extreme phenotype where somite boundaries have been eliminated on the injected side (1 ng xBmal1 MO). Panels C (uninjected) and E (injected) show effects on somite size, organization and boundaries in an embryo injected with 1 ng (xBmal1 MO). Expression from the uninjected side of the embryo can be seen through the cleared embryo in E, grey dots separate foreground image from the background. Panels D and F show similar results were observed with injection of xNocturnin MO (1 ng) although there is usually less disruption of somite borders (D, uninjected; F, xNocturnin MO injected). All images were taken at the same magnification (scale bar = 10 µm). doi:10.1371/journal.pone.0108266.g004 Image published in: Curran KL et al. (2014) Image reproduced on Xenbase with permission of the publisher and the copyright holder. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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