XB-IMG-133440
Xenbase Image ID: 133440
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FIGURE 2.
Overexpression of xdpcp leads to defects in mesendoderm formation. A, xdpcp RNA (2 ng)-injected embryos show mesodermal defective phenotype. B, dorsal injection of xdpcp RNA inhibits the expression of endogenous mesodermal markers such as xbra, chordin, and goosecoid (gsc). Four-cell stage embryos were injected into the dorsal regions with 2 ng of xdpcp RNA or none, cultured until stage 10, and then subjected to in situ hybridization. C and D, RT-PCR analysis revealing that Xdpcp inhibits the expression of mesendodermal markers induced by activin in animal cap tissues, whereas it does not affect the expression of the Bmp target genes. +RT and −RT, control RT-PCR on the whole embryo RNA in the presence or absence of reverse transcriptase; AC, uninjected animal cap cells; (−), the animal cap cells injected with activin-βB or bmp4 RNA alone. Ornithine decarboxylase (ODC) serves as a loading control. E and F, luciferase assays in Xenopus embryos injected with combinations of indicated reagents. The amount of injected reagents is as follows: activin-βB RNA, 5 pg; Xnr-1 RNA, 100 pg; xdpcp RNA, 2 ng; ARE-Luc reporter DNA, 40 pg; 6DE-Luc reporter DNA, 40 pg. Error bars represent the standard deviation. Image published in: Cheong SM et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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