Xenbase Image ID: 133444

FIGURE 6. Xdpcp inhibits the interaction between activin receptor and Smad2. A, Xdpcp interacts with type I activin receptor, Alk4. Xenopus embryo lysates injected with combinations of Myc-tagged xdpcp RNA (1 ng) and HA-tagged Alk4 RNA (1 ng) as indicated were immunoprecipitated with anti-HA antibody and then subjected to immunoblotting (IB) analysis. Asterisk represents the constitutively active form of Alk4. B, Xdpcp does not bind to Alk3, the Bmp receptor. HEK293FT cell lysates transfected with combinations of the indicated expression plasmids for GFP-tagged Xdpcp, HA-tagged Alk4, and HA-tagged Alk3 protein were immunoprecipitated with anti-HA antibody, and subsequently subjected to immunoblotting analysis with anti-GFP antibody to detect their association. C, the association between Alk4 and Smad2 is inhibited by coexpression of xdpcp in Xenopus embryos. The amount of injected reagents is as follows: activin-βB, 5 pg; GFP-tagged Xdpcp, 2 ng; HA-tagged Alk4, 1 ng; Myc-tagged Smad2, 1 ng. D, the L45 region of Alk4 is required for binding to Xdpcp. The cells expressing the indicated reagents were subjected to immunoprecipitation (IP) and Western blot analysis after a 1-h TGF-β treatment. Image published in: Cheong SM et al. (2009) Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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