XB-IMG-133466
Xenbase Image ID: 133466
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Figure 2. A fluorometric assay for the Chordinase activity of Tolloids shows that BMP4 is a specific inhibitor of the reaction. (A) A new fluorogenic peptide substrate for Tolloids containing the sequence of the Xenopus Chordin cleavage site. (B) The Chd peptide substrate is efficiently digested by BMP1 enzyme, a reaction that is competitively inhibited by 60 nM full-length Chordin proteins. The Ki (17 nM) is similar to the Km of tolloids for full-length chordin (Lee et al. 2006). (C) BMP4, but not other regulators of D–V patterning, inhibits BMP1 metalloproteinase activity. All proteins were added at 120 nM final concentration. Image published in: Lee HX et al. (2009) Copyright © 2009. Image reproduced on with permission of the Publisher, Cold Spring Harbor Laboratory Press. This is an Open Access article. Larger Image Printer Friendly View |