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Fig. 4. Co-expression of ZFPIPp- FLAG or N-ZFPIP-FLAG and Pbx1b-HA in Cos-7 cells. Immunocytochemistry assays were carried out using a-HA and a-FLAG antibodies on cells expressing independently or co-expressing ZFPIP proteins (ZFPIPp-FLAG or N-ZFPIPFLAG) and Pbx1b-HA. ZFPIPp-FLAG, N-ZFPIP-FLAG and Pbx1b-HAproteins were observed, respectively, in the nucleus and in the cytoplasm when they were expressed separately. In contrast, co-expression in the same cell type of ZFPIPp or N-ZFPIP and Pbx1b, led to cytoplasmic and perinuclear localization of ZFPIP ⁄ Pbx1 complexes.

Image published in: Laurent A et al. (2009)

Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons.

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