XB-IMG-133539
Xenbase Image ID: 133539
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Fig. 2. Inductive activities of X-bhh-derived
polypeptides in animal cap explants.
Animal caps from embryos injected with
various RNAs were cultured until sibling
embryos had reached stage 25, at which
time samples were processed for RT-PCR
for the markers shown. Minus (-) lanes are
controls omitting reverse transcriptase in
the first strand synthesis, and plus (+) lanes
contain reverse transcriptase. Lanes 1, 2:
stage 25 embryos as positive controls.
Lanes 3, 4: animal caps from uninjected
embryos as negative controls. Lanes 5, 6:
animal caps from embryos injected with Xbhh
RNA. Lanes 7, 8: animal caps from
embryos injected with N. Lanes 9, 10:
animal caps from embryos injected with
UHA. Lanes 11, 12: animal caps from
embryos injected with a frame-shifted
version of X-shh (X-shhfs) as a negative
control. Lanes 13, 14: Animal caps from
embryos injected with noggin RNA for
comparison of induced neural markers.
Note the high-level induction by active
constructs (X-bhh, N, and UHA) of the
cement gland marker XAG-1 and the
relatively lower level induction of the
anterior neural markers XANF-2 and Otx-A,
without induction of the general neural
marker, N-CAM. Image published in: Lai CJ et al. (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |