XB-IMG-133542
Xenbase Image ID: 133542
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Fig. 5. DN-C interferes with X-bhh and N activity in animal cap
explants. Embryos were injected with various RNAs, animal cap
explants were cultured until sibling embryos reached tailbud (stage
25), at which time RT-PCR was used to analyze the expression of the
cement gland marker XAG-1 and the control RNA, EF-1a. Lanes 1,
2: control animal caps from uninjected embryos. Lanes 3, 4: animal
caps from embryos injected with both X-bhh and prolactin RNAs.
Lanes 5, 6: animal caps from embryos injected with both X-bhh and
DN-C. Lanes 7, 8: animal caps from embryos injected with both N
and prolactin RNAs. Lanes 9, 10: animal caps from embryos injected
with both N and DN-C. The N and X-bhh experiments were
conducted independently and thus absolute levels in lanes 3-6 should
not be compared to those in lanes 7-10. Note that the induction of
XAG-1 expression by X-bhh or N is reduced by co-injection of DN-C. Image published in: Lai CJ et al. (1995) Copyright © 1995. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |