XB-IMG-133551
Xenbase Image ID: 133551
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Fig. 2. Reverse transcription–polymerase chain reaction (RT–PCR)
of normal mouse IgG (NMI)- or anti-Xtr-coimmunoprecipitated
mRNAs from the supernatant of centrifuged eggs using oligo dT
primer for RT and gene-specific primers (RCC1, XRHAMM,
XL-INCENP, XMad2, XDead end, and SP4) for PCR. W: Total RNA
(1 lg) from fertilized eggs (30 min after insemination) was used
for RT–PCR. Spermatogenic cell-specific SP4 mRNA was
added to the immunoprecipitates with NMI (NMI) and anti-Xtr
mAb (a Xtr) for comparing the recovery of RNA during
purification process and efficiency of RT–PCR between the
immunoprecipitates. Image published in: Golam Mostafa M et al. (2009) Copyright © 2009. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |