Xenbase Image ID: 133614

Figure 2 External phenotypes of Pax6, mNcad, Ncad, and NCAM knockdown embryos. A: Representative tadpoles at Stage 45 carrying control morpholino (CO MO) or antisense morpholino against Pax6 (Pax6 MO) or individual Pax6-de- pendent genes coding for the cell adhesion molecules (mNcad MO, Ncad MO, or NCAM MO). Pax6 MO, two small-eyed Pax6-deficient tadpoles and one \eyeless" indi- vidual. Note the delay in differentiation of the intestinal tract, the most severe case presenting prolapse (arrow). mNcad MO, two small-eyed individuals with delayed differentiation of the intestinal tract. Ncad MO, two small-eyed individuals with delayed differentiation including the intestinal tract. NCAM MO, tadpole with slightly decreased eye size and normal differentiation of the body. B: Normal neurula (CO MO) and two arrested gastrulae (mNcad MO) with dissociat- ing cells in one body half, resulting from injection of 15 ng of morpholino directed against mNcad into one cell of the two-cell stage. C: Frequency of external phenotypes, scored at Stage 42. Averages of 3–6 experimental series with >100 injected embryos. Embryos were injected with no (none), control (CO) or with antisense morpholino (MO) against Pax6, mNcad, Ncad, or NCAM. OK, normal embryos; eye, reduced or rudimentary eyes; def, various deformities (microcephaly, axial disturbance, bent tail) considered to be non-specific; diss, entirely dissociated embryos, most often, accompanied by disturbances in the digestive tract; dead, le- thal before Stage 42. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.] Image published in: Rungger-Brändle E et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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