Xenbase Image ID: 133619

Figure 7 Rescue of target gene expression by Pax6. A: Steady state Pax6, mNcad, Ncad, and NCAM mRNA levels in embryos (Stage 30) carrying either control (MO-CO) or anti-Pax6 morpholino (MO-Pax6), mixed with a CMV- driven expression vector coding for either red fluorescent protein (CMV-Red) or Pax6 (CMV-Pax6). B: Frequency of phenotypes (Stage 36) in sibling embryos (treated as in A). OK, normal; eye, small or defective eye; def, other malfor- mations. C: Western blots for mNcad and bactin in knock- down and rescued embryos (as in A, Stage 30). The smaller reactive band is a degradation product. D: mNcad mRNA levels in embryos (Stage 30) carrying anti-Pax6 (MO-Pax6) or anti-mNcad morpholino (MO-mNcad) and CMV-Red or CMV-mNcad vector. E: Frequency of phenotypes in sibling embryos (Stage 36; treated as in D, phenotypes as in B). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.] Image published in: Rungger-Brändle E et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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