Xenbase Image ID: 133620

Figure 8 Chromatin immunoprecipitation of Pax6-binding motifs in genes coding for cell adhesion molecules. A: Sketch of promoter regions of the genes coding for mNcad, Ncad, and NCAM. Hypothetical Pax6-binding sites, revealed by computer search, are indicated by circles of varying diameter, reflecting their respective scores. Amplicons (boxes) of pro- moter regions (PRO) or an intragenic region (CO) may origi- nate from DNA fragments extending 250–500 bp (lozenge) on either side. Repetitive elements (stippled line) and so far not sequenced segments (N) preclude analysis of the proximal promoter region of mNcad. B: ChIP analysis of the four gene regions mNcad PRO and CO, Ncad PRO and NCAM PRO, with histone H3 antibodies yields near-equal values in both head and tail chromatin, expressed as percent of the input ma- terial precipitated. Average from two experiments 6 SD. C: ChIP analysis with anti-Clock and anti-Pax6 antibodies on the same gene regions as in B. Note that about twice as many fragments of the promoter regions of the three genes coding for cell adhesion proteins were precipitated from chromatin preparations of heads by Pax6 antibody as compared to the nonspecific anti-Clock antibody. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary. com.] Image published in: Rungger-Brändle E et al. (2010) Copyright © 2010. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View

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