Xenbase Image ID: 133670

Fig. 2. Overexpressed BMP-GFP fusion proteins diffuse in Brachet’s cleft. (A and B) Dorsally expressed zADMP-GFP and xBMP2-GFP fusion proteins diffuse within the narrow confines of Brachet’s cleft (marked by arrowheads) away from the point of mRNA injection in ectodermal cells (indicated by a red dotted line) (n = 7 and n = 6, respectively). GFP fusion proteins were detected by GFP immunostainings of transverse optical sections of stage-12 embryos through the animal–vegetal axis. (C) Diagram of stage-12 sagittal section of a Xenopus embryo (after P. Nieuwkoop, ref. 38). Brachet’s cleft is the narrow cavity that separates the mesodermal and anterior endodermal layers from the ectoderm and encircles the entire D-V axis. Image published in: Plouhinec JL et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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