XB-IMG-133671
Xenbase Image ID: 133671
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Fig. 3. Endogenous Chordin protein diffusion within Brachet’s cleft compared with the expression of chordin mRNA. (A and B) Immunostainings of Chordin protein in late gastrula (stage 12) embryos in transverse (n = 16) or sagittal (n = 15) sections, respectively, using an affinity-purified Chordin antibody. Chordin protein is detected throughout the entire length of Brachet’s cleft (arrowheads) forming a gradient from dorsal toward ventral. Chd, Chordin. (C) Imaging of the Chordin gradient following the entire circumference in a clockwise manner (circular arrow) or in a radial manner (numbered arrows). (D) Measurement of the Chordin gradient of the embryo seen in C. Note that the gradient forms over a very long distance of almost 2 mm; similar profiles were obtained in the four embryos analyzed. (E) Intensity of fluorescence plotted along five radial lines from central to peripheral. Line 1 is dorsal and shows Chordin protein peaks in organizer mesoderm and in Brachet’s cleft. In the other lines a peak is seen in Brachet’s cleft, even in the ventral-most region. (F and G) In situ hybridization of stage-12 embryo in transverse or sagittal section showing that chordin mRNA is transcribed only in the dorsal side (arrowheads); compare with the panels (A and B) showing Chordin protein localization at the same stage. Image published in: Plouhinec JL et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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