XB-IMG-133673
Xenbase Image ID: 133673
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Fig. 5. The Chordin and pSmad1/5/8 gradients self-regulate in dorsal half-embryos. The ventral side of albino embryos was marked at the 8-cell stage using the method described in Fig. S4. Embryos were bisected at stage 9, cultured until stage 12, fixed, and sectioned transversely. All embryos were siblings and images were processed identically. (A–C) pSmad1/5/8 immunostaining of whole embryo (A, n = 3), dorsal (B, n = 6) or ventral (C, n = 3 of 4 showing the phenotype) half-embryos. Note that the BMP gradient was reestablished in the dorsal half-embryo, while very strong uniform pSmad1/5/8 was present in the ventral half-embryo. (D–F) Chordin immunostaining of whole embryo (D, n = 3), dorsal (E, n = 10) or ventral (F, n = 7) half-embryo. The Chordin gradient was regenerated in the Brachet’s cleft of dorsal half-embryos (in 8 of 10), while no Chordin expression was detected in the ECM of the ventral half-embryo (in 6 of 7). Similar results were obtained in two independent experiments. Image published in: Plouhinec JL et al. (2013) Copyright © 2013. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |